Journal: PLOS One

Article Title: KIF18A induces the EMT process of hepatoma cells through the 5-LOX-dependent arachidonic acid pathway

doi: 10.1371/journal.pone.0333385

Figure Lengend Snippet: (A) Search for the relative expression levels of different KIF18A in liver cancer cells in the online database ( https://sites.broadinstitute.org/ccle/ ). (B) The relative expression levels of mRNA in normal liver cell THLE-2 and liver cancer cells SNU423, JHH2, JHH7, HepG2, and LI7 were detected by RT-qPCR. (C) After transfection with the overexpression KIF18A vector for 48 h in liver cancer HepG2 cells, the relative expression level of KIF18A mRNA was detected by RT-qPCR. (D) The relative expression level of KIF18A protein was detected by western blotting in HepG2 cells. (E) Column diagram of the relative expression level of KIF18A in HepG2 cells compared to GAPDH by western blotting. (F) After transfection with the overexpression KIF18A vector for 48 h in liver cancer SNU423 cells, the relative expression level of KIF18A mRNA was detected by RT-qPCR. (G) Column diagram of the relative expression level of KIF18A compared to GAPDH by western blotting. (H) Column diagram of the relative expression level of KIF18A in SNU423 cells compared to GAPDH by western blotting. (I) Changes in cell viability were detected by CCK8 after transfection with the KIF18A overexpression vector in HepG2 cells and addition of U73122 after incubation for 24 h. (J) Changes in cell viability were detected by CCK8 after transfection with the KIF18A interfering RNA in SNU423 cells. (Compared between the two groups, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: The human liver normal cell line THLE-2 and the human liver cancer cell lines HepG2, SNU423, JHH2, JHH7, and LI7 were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Quantitative RT-PCR, Transfection, Over Expression, Plasmid Preparation, Western Blot, Incubation

Journal: PLOS One

Article Title: KIF18A induces the EMT process of hepatoma cells through the 5-LOX-dependent arachidonic acid pathway

doi: 10.1371/journal.pone.0333385

Figure Lengend Snippet: (A) Transfection with KIF18 overexpression vector and interfering RNA, and U73122 was added after overexpression vector transfection. Changes in the migration ability of liver cancer cells HepG2 and SNU423 were detected by cell scratch assay. (B) Changes in the invasion ability of liver cancer cells HepG2 and SNU423 were detected by Transwell assay. (Compared between the two groups, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: The human liver normal cell line THLE-2 and the human liver cancer cell lines HepG2, SNU423, JHH2, JHH7, and LI7 were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Transfection, Over Expression, Plasmid Preparation, Migration, Wound Healing Assay, Transwell Assay

Journal: PLOS One

Article Title: KIF18A induces the EMT process of hepatoma cells through the 5-LOX-dependent arachidonic acid pathway

doi: 10.1371/journal.pone.0333385

Figure Lengend Snippet: (A) Detection of the localization and expression level changes of E-cadherin in HepG2 cells by immunofluorescence. (B) Detection of the localization and expression level changes of N-cadherin in HepG2 cells by immunofluorescence. (C) Relative expression level of E-cadherin relative to DAPI fluorescence intensity. (D) Relative expression level of N-cadherin relative to DAPI fluorescence intensity. (E) Detection of the relative expression levels of E-cadherin, N-cadherin, Snail1, and Vimentin proteins in HepG2 cells by western blotting. (F) Detection of the localization and expression level changes of E-cadherin in SNU423 cells by immunofluorescence. (G) Detection of the localization and expression level changes of N-cadherin in SNU423 cells by immunofluorescence. (H) Relative expression level of E-cadherin relative to DAPI fluorescence intensity. (I) Relative expression level of N-cadherin relative to DAPI fluorescence intensity. (J) Detection of the relative expression levels of E-cadherin, N-cadherin, Snail1, and Vimentin proteins in SNU423 cells by western blotting. (Compared between the two groups, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: The human liver normal cell line THLE-2 and the human liver cancer cell lines HepG2, SNU423, JHH2, JHH7, and LI7 were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Immunofluorescence, Fluorescence, Western Blot

Journal: PLOS One

Article Title: KIF18A induces the EMT process of hepatoma cells through the 5-LOX-dependent arachidonic acid pathway

doi: 10.1371/journal.pone.0333385

Figure Lengend Snippet: (A) Detection of the relative expression level of 5-LOX protein in HepG2 cells by western blotting. (B) Detection of the relative expression level of 5-LOX protein in SNU423 cells by western blotting. (C) Relative expression levels of 5-HETE, 12-HETE, 15-HETE, LTB4, LTC4, LTD4, and AA in the cell supernatant of HepG2 cells were detected by ELISA. (D) Relative expression levels of 5-HETE, 12-HETE, 15-HETE, LTB4, LTC4, LTD4, and AA in the cell supernatant of SNU423 cells were detected by ELISA. (E) In SNU423 cells, after knocking down KIF18A, 5 – LOX was overexpressed, and the change in the expression level of 5 – LOX protein was detected by Western blot assay. (F) In SNU423 cells, following the knockdown of KIF18A and subsequent overexpression of 5 – LOX, the relative expression levels of 5 – HETE, 12 – HETE, 15 – HETE, LTB4, LTC4, LTD4 and AA in the supernatant of SNU423 cells were measured by ELISA.(Compared between the two groups, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: The human liver normal cell line THLE-2 and the human liver cancer cell lines HepG2, SNU423, JHH2, JHH7, and LI7 were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Knockdown, Over Expression

Journal: Journal of ethnopharmacology

Article Title: Exploring the dietary and therapeutic potential of licorice (Glycyrrhiza uralensis Fisch.) sprouts.

doi: 10.1016/j.jep.2024.118101

Figure Lengend Snippet: Fig. 1. MTT assay for the determination of HepG2 cell viability.MTT assay was performed to determine the viability of HepG2 cells after 24 h of treatment with aqueous and organic extracts of Licorice (Glycyrrhiza uralensis) seeds (control) and sprouts. Each data point represents the average of at least three replicates and error bars represent the standard deviation.

Article Snippet: The human liver cancer cell line (HepG2) was acquired from Korean Cell Line Bank (KCLB stock No. 88065).

Techniques: MTT Assay, Control, Standard Deviation

Journal: Journal of ethnopharmacology

Article Title: Exploring the dietary and therapeutic potential of licorice (Glycyrrhiza uralensis Fisch.) sprouts.

doi: 10.1016/j.jep.2024.118101

Figure Lengend Snippet: Fig. 2. Inhibition of the intracellular lipids, triglycerides and cholesterol by licorice extracts. Oleic acid and Palmitic acid were used to mimic non-alcoholic FALD in HepG2 cells for 24 h. Licorice aqueous and organic extracts were added for 24 h to determine their ability of reducing the available lipids (A), triglycerides (B), and cholesterol (C). HepG2 cells only, HepG2 supplemented with only OA + PA, and HepG2 cells supplemented with OA + PA and 5′-AMP activated protein kinase were used as comparative controls. Furthermore, HepG2 cells supple mented with H2O2 only and those supplemented with vitamin C were used as comparative controls to measure cellular lipid peroxidation (D). Each data point represents the average of at least three replicates and error bars represent the standard deviation.

Article Snippet: The human liver cancer cell line (HepG2) was acquired from Korean Cell Line Bank (KCLB stock No. 88065).

Techniques: Inhibition, Standard Deviation